Applications of target prioritization

TargetMine has been successfully applied to several instances.

Here are some applications which use TargetMine for analysis.


Example 1

click on the figure for details. (External link to TargetMine)


Fifty-six proteins were identified by yeast 2-hybrid screening. The proteins were combined with their interacting partners to construct the extended network. The four prioritized targets are enolase (ENO1), paxillin (PXN), SLC25A5 and vinculin (VCL). Fifty-six proteins were identified by yeast 2-hybrid screening. The proteins were combined with their interacting partners to construct the extended network. The four prioritized targets are enolase (ENO1), paxillin (PXN), SLC25A5 and vinculin (VCL).


Example 2

click on the figure for details. (External link to TargetMine)


In order to understand the roles of PA28-gamma in HCV infection, we compared and investigated the levels of cellular proteins in transgenic mice expressing HCV core protein in the liver (an in vivo model of HCV pathogenesis) With or without knock out of PA28-gamma expression. In order to understand the roles of PA28-gamma in HCV infection, we compared and investigated the levels of cellular proteins in transgenic mice expressing HCV core protein in the liver (an in vivo model of HCV pathogenesis) With or without knock out of PA28-gamma expression.

More examples could be found in the following publications.


References:

  • Nakae Y, et al. (2015) Two distinct effector memory cell populations of WT1 (Wilms’ tumor gene 1)-specific cytotoxic T lymphocytes in acute myeloid leukemia patients. Cancer Immunol Immunother. 64(7):791-804. [PubMed:25835542]
  • Camargo LM, et al. (2015) Pathway-based analysis of genome-wide siRNA screens reveals the regulatory landscape of APP processing. Plos ONE 10(2):e0115369. [PubMed:25723573]
  • Tripathi LP, et al. (2013) Understanding the Biological Context of NS5A-Host Interactions in HCV Infection: A Network-Based Approach. J Proteome Res. [PubMed:23682656]
  • Ihara S, et al. (2012) Inhibitory Roles of Signal Transducer and Activator of Transcription 3 in Antitumor Immunity during Carcinogen-Induced Lung Tumorigenesis. Cancer Res. 72(12):2990-2999. [PubMed:22659452]
  • Tripathi LP, et al. (2012) Proteomic Analysis of Hepatitis C Virus (HCV) Core Protein Transfection and Host Regulator PA28γ Knockout in HCV Pathogenesis: A Network-Based Study. J Proteome Res. 11: 3664-3679. [PubMed:22646850]
  • Tripathi LP, et al. (2010) Network based analysis of hepatitis C virus Core and NS4B protein interactions. Mol Biosyst 6: 2539-2553. [PubMed:20953506]